A replicating stem-like cell that contributes to bone morphogenetic protein 2-induced heterotopic bone formation.

ABSTRACT: Bone morphogenetic protein 2 (BMP2)-induced heterotopic bone formation (HBF) starts synchronously from zero upon BMP2 induction, which is advantageous for lineage tracking. The studies reported here in GLAST-Cre^Ert2 :tdTomato red (TR)^floxSTOPflox mice during BMP2-induced HBF show 78.8 ± 11.6% of chondrocytes and 86.5 ± 1.9% of osteoblasts are TR⁺ after approximately 1 week. Clustering after single-cell RNAseq resulted in nine cell types, and analysis revealed one as a highly replicating stem-like cell (RSC). Pseudotiming suggested that the RSC transitions to a mesenchymal stem-like cell that simultaneously expresses multiple osteoblast and chondrocyte transcripts (chondro-osseous progenitor [COP]). RSCs and COPs were isolated using flow cytometry for unique

SUBMITTER: Mejia J 

PROVIDER: S-EPMC7980206 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature