Stochastic combinations of actin regulatory proteins are sufficient to drive filopodia formation.

ABSTRACT: Assemblies of actin and its regulators underlie the dynamic morphology of all eukaryotic cells. To understand how actin regulatory proteins work together to generate actin-rich structures such as filopodia, we analyzed the localization of diverse actin regulators within filopodia in Drosophila embryos and in a complementary in vitro system of filopodia-like structures (FLSs). We found that the composition of the regulatory protein complex where actin is incorporated (the filopodial tip complex) is remarkably heterogeneous both in vivo and in vitro. Our data reveal that different pairs of proteins correlate with each other and with actin bundle length, suggesting the presence of functional subcomplexes. This is consistent with a theoretical framework where three or more redundant subcomplex

SUBMITTER: Dobramysl U 

PROVIDER: S-EPMC7980258 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature