Dual-process brain mitochondria isolation preserves function and clarifies protein composition.

ABSTRACT: The brain requires continuously high energy production to maintain ion gradients and normal function. Mitochondria critically undergird brain energetics, and mitochondrial abnormalities feature prominently in neuropsychiatric disease. However, many unique aspects of brain mitochondria composition and function are poorly understood. Developing improved neuroprotective therapeutics thus requires more comprehensively understanding brain mitochondria, including accurately delineating protein composition and channel-transporter functional networks. However, obtaining pure mitochondria from the brain is especially challenging due to its distinctive lipid and cell structure properties. As a result, conflicting reports on protein localization to brain mitochondria abound. Here we illustrate this p

SUBMITTER: Noterman MF 

PROVIDER: S-EPMC7980376 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature