Zn²⁺ influx activates ERK and Akt signaling pathways.

ABSTRACT: Zinc (Zn²⁺) is an essential metal in biology, and its bioavailability is highly regulated. Many cell types exhibit fluctuations in Zn²⁺ that appear to play an important role in cellular function. However, the detailed molecular mechanisms by which Zn²⁺ dynamics influence cell physiology remain enigmatic. Here, we use a combination of fluorescent biosensors and cell perturbations to define how changes in intracellular Zn²⁺ impact kinase signaling pathways. By simultaneously monitoring Zn²⁺ dynamics and kinase activity in individual cells, we quantify changes in labile Zn²⁺ and directly correlate changes in Zn²⁺ with ERK and Akt activity. Under our experimental conditions, Zn²⁺ fluctuations are not toxic

SUBMITTER: Anson KJ 

PROVIDER: S-EPMC7980380 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature