Quantification of Brucella abortus population structure in a natural host.

ABSTRACT: Cattle are natural hosts of the intracellular pathogen Brucella abortus, which inflicts a significant burden on the health and reproduction of these important livestock. The primary routes of infection in field settings have been described, but it is not known how the bovine host shapes the structure of B. abortus populations during infection. We utilized a library of uniquely barcoded B. abortus strains to temporally and spatially quantify population structure during colonization of cattle through a natural route of infection. Introducing 10⁸ bacteria from this barcoded library to the conjunctival mucosa resulted in expected levels of local lymph node colonization at a 1-wk time point. We leveraged variance in strain abundance in the library to demonstrate

SUBMITTER: Fiebig A 

PROVIDER: S-EPMC7980399 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature