New sources of lentil germplasm for aluminium toxicity tolerance identified by high throughput hydroponic screening.

ABSTRACT: Aluminium (Al) toxicity in acid soils inhibits root elongation and development causing reduced water and nutrient uptake by the root system, which ultimately reduces the crop yield. This study established a high throughput hydroponics screening method and identified Al toxicity tolerant accessions from a set of putative acid tolerant lentil accessions. Four-day old lentil seedlings were screened at 5 µM Al (pH 4.5) for three days in hydroponics. Measured pre and post treatment root length was used to calculate the change in root length (ΔRL) and relative root growth (RRG%). A subset of 15 selected accessions were used for acid soil Al screening, and histochemical and biochemical analyses. Al treatment significantly reduced the ΔRL with an average of 32.3% reduction observed compared to the

SUBMITTER: Kulkarni V 

PROVIDER: S-EPMC7981344 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature