Comparative transcriptomic analysis of the different developmental stages of ovary in red swamp crayfish Procambarus clarkii.

ABSTRACT:

Background

The red swamp crayfish Procambarus clarkii is a freshwater species that possesses high adaptability, environmental tolerance, and fecundity. P. clarkii is artificially farmed on a large scale in China. However, the molecular mechanisms of ovarian development in P. clarkii remain largely unknown. In this study, we identified four stages of P. clarkii ovary development, the previtellogenic stage (stage I), early vitellogenic stage (stage II), middle vitellogenic stage (stage III), and mature stage (stage IV) and compared the transcriptomics among these four stages through next-generation sequencing (NGS).

Results

The total numbers of clean reads of the four stages ranged from 42,013,648 to 62,220,956. A total of 216,444 unigenes were obtained, and the GC content of

SUBMITTER: Zhong Y 

PROVIDER: S-EPMC7981885 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature