Molecular barcode and morphological analysis of Smilax purhampuy Ruiz, Ecuador.

ABSTRACT: Smilax plants are distributed in tropical, subtropical, and temperate regions in both hemispheres of the world. They are used extensively in traditional medicines in a number of countries. However, morphological and molecular barcodes analysis, which may assist in the taxonomic identification of species, are lacking in Ecuador. In order to evaluate the micromorphological characteristics of these plants, cross sections of Smilax purhampuy leaves were obtained manually. The rhizome powder, which is typically used in traditional medicines, was analyzed for micromorphological characteristics. All samples were clarified with 1% sodium hypochlorite. Tissues were colored with 1% safranin in water and were fixed with glycerinated gelatin. DNA was extracted from the leaves using a modified C

SUBMITTER: Soledispa P 

PROVIDER: S-EPMC7982074 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature