The role of M1 and M2 macrophage polarization in progression of medication-related osteonecrosis of the jaw.

ABSTRACT:

Objectives

The aim of this study was to investigate the relationship between M1 and M2 macrophage polarization and clinical stage in patients with medication-related osteonecrosis of the jaw (MRONJ) who underwent treatment with bisphosphonates or denosumab.

Materials and methods

M1 and M2 macrophage density and expression of interleukin (IL)-6 and IL-10 were assessed on biopsies of mucosal tissues surrounding necrotic bone in 30 MRONJ patients with stages 1-3 and controls. For identification of M1 and M2 macrophages, double CD68/iNOS and CD68/CD206 immunofluorescence staining was conducted, respectively. Computer-assisted immunofluorescence quantification of markers was performed.

Results

Early stage 1 MRONJ patients showed a switch toward the M2 phenotype, as indicat

SUBMITTER: Paschalidi P 

PROVIDER: S-EPMC7982347 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature