Mechanism of succinate efflux upon reperfusion of the ischaemic heart.

ABSTRACT:

Aims

Succinate accumulates several-fold in the ischaemic heart and is then rapidly oxidized upon reperfusion, contributing to reactive oxygen species production by mitochondria. In addition, a significant amount of the accumulated succinate is released from the heart into the circulation at reperfusion, potentially activating the G-protein-coupled succinate receptor (SUCNR1). However, the factors that determine the proportion of succinate oxidation or release, and the mechanism of this release, are not known.

Methods and results

To address these questions, we assessed the fate of accumulated succinate upon reperfusion of anoxic cardiomyocytes, and of the ischaemic heart both ex vivo and in vivo. The release of accumulated succinate was selective and was enhanced by acidifica

SUBMITTER: Prag HA 

PROVIDER: S-EPMC7983001 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature