Mechanism and functional role of the interaction between CP190 and the architectural protein Pita in Drosophila melanogaster.

ABSTRACT:

Background

Pita is required for Drosophila development and binds specifically to a long motif in active promoters and insulators. Pita belongs to the Drosophila family of zinc-finger architectural proteins, which also includes Su(Hw) and the conserved among higher eukaryotes CTCF. The architectural proteins maintain the active state of regulatory elements and the long-distance interactions between them. In particular, Pita is involved in the formation of several boundaries between regulatory domains that controlled the expression of three hox genes in the Bithorax complex (BX-C). The CP190 protein is recruited to chromatin through interaction with the architectural proteins.

Results

Using in vitro pull-down analysis, we precisely mapped two unstructured regions of Pita that

SUBMITTER: Sabirov M 

PROVIDER: S-EPMC7983404 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature