JAK2 regulates Nav1.6 channel function via FGF14^Y158 phosphorylation.

ABSTRACT:

Background

Protein interactions between voltage-gated sodium (Nav) channels and accessory proteins play an essential role in neuronal firing and plasticity. However, a surprisingly limited number of kinases have been identified as regulators of these molecular complexes. We hypothesized that numerous as-of-yet unidentified kinases indirectly regulate the Nav channel via modulation of the intracellular fibroblast growth factor 14 (FGF14), an accessory protein with numerous unexplored phosphomotifs and required for channel function in neurons.

Methods

Here we present results from an in-cell high-throughput screening (HTS) against the FGF14: Nav1.6 complex using >3000 diverse compounds targeting an extensive range of signaling pathways. Regulation by top kinase targets was then

SUBMITTER: Wadsworth PA 

PROVIDER: S-EPMC7984254 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature