Fetal mitochondrial DNA in maternal plasma in surrogate pregnancies: Detection and topology.

ABSTRACT:

Objectives

Due to the maternally-inherited nature of mitochondrial DNA (mtDNA), there is a lack of information regarding fetal mtDNA in the plasma of pregnant women. We aim to explore the presence and topologic forms of circulating fetal and maternal mtDNA molecules in surrogate pregnancies.

Methods

Genotypic differences between fetal and surrogate maternal mtDNA were used to identify the fetal and maternal mtDNA molecules in plasma. Plasma samples were obtained from the surrogate pregnant mothers. Using cleavage-end signatures of BfaI restriction enzyme, linear and circular mtDNA molecules in maternal plasma could be differentiated.

Results

Fetal-derived mtDNA molecules were mainly linear (median: 88%; range: 80%-96%), whereas approximately half of the maternal-deriv

SUBMITTER: Ma ML 

PROVIDER: S-EPMC7984455 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature