Age-related changes in polycomb gene regulation disrupt lineage fidelity in intestinal stem cells.

ABSTRACT: Tissue homeostasis requires long-term lineage fidelity of somatic stem cells. Whether and how age-related changes in somatic stem cells impact the faithful execution of lineage decisions remains largely unknown. Here, we address this question using genome-wide chromatin accessibility and transcriptome analysis as well as single-cell RNA-seq to explore stem-cell-intrinsic changes in the aging Drosophila intestine. These studies indicate that in stem cells of old flies, promoters of Polycomb (Pc) target genes become differentially accessible, resulting in the increased expression of enteroendocrine (EE) cell specification genes. Consistently, we find age-related changes in the composition of the EE progenitor cell population in aging intestines, as well as a significant increase in th

SUBMITTER: Tauc HM 

PROVIDER: S-EPMC7984841 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature