Damage-free light-induced assembly of intestinal bacteria with a bubble-mimetic substrate.

ABSTRACT: Rapid evaluation of functions in densely assembled bacteria is a crucial issue in the efficient study of symbiotic mechanisms. If the interaction between many living microbes can be controlled and accelerated via remote assembly, a cultivation process requiring a few days can be ommitted, thus leading to a reduction in the time needed to analyze the bacterial functions. Here, we show the rapid, damage-free, and extremely dense light-induced assembly of microbes over a submillimeter area with the "bubble-mimetic substrate (BMS)". In particular, we successfully assembled 10⁴-10⁵ cells of lactic acid bacteria (Lactobacillus casei), achieving a survival rate higher than 95% within a few minutes without cultivation process. This type of light-induced assembly on substrates

SUBMITTER: Hayashi K 

PROVIDER: S-EPMC7985151 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature