Methylation analysis of Klebsiella pneumoniae from Portuguese hospitals.

ABSTRACT: Klebsiella pneumoniae is an important nosocomial infectious agent with a high antimicrobial resistance (AMR) burden. The application of long read sequencing technologies is providing insights into bacterial chromosomal and putative extra-chromosomal genetic elements (PEGEs) associated with AMR, but also epigenetic DNA methylation, which is thought to play a role in cleavage of foreign DNA and expression regulation. Here, we apply the PacBio sequencing platform to eight Portuguese hospital isolates, including one carbapenemase producing isolate, to identify methylation motifs. The resulting assembled chromosomes were between 5.2 and 5.5Mbp in length, and twenty-six PEGEs were found. Four of our eight samples carry bla_CTX-M-15, a dominant Extended Spectrum Beta Lactamase in Europe

SUBMITTER: Spadar A 

PROVIDER: S-EPMC7985491 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature