Sperm DNA Methylation at Metabolism-Related Genes in Vegan Subjects.

ABSTRACT:

Objective

To investigate if epigenome of sperm cells could be dynamically affected by nutrition.

Design and methods

We assessed 40 healthy volunteers with different dietary habits and collected their demographic characteristics, as well as clinical and anthropometric parameters. We compared methylation profiles in sperm quantified by bisulfite pyrosequencing, at promoter-associated CpG sites of genes involved in metabolism including fat mass and obesity-associated (FTO) and melanocortin-4 receptor (MC4R) from six vegans and 34 omnivores. In addition, the FTO rs9939609 (T>A) was genotyped.

Results

Higher DNA methylation levels were detected in the sperm of vegan at FTO gene CpG1 (p=0.02), CpG2 (p=0.001), CpG3 (p=0.004), and CpG4 (p=0.003) si

SUBMITTER: Franzago M 

PROVIDER: S-EPMC7985526 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature