MicroRNA‑1271‑5p alleviates the malignant development of hepatitis B virus‑mediated liver cancer via binding to AQP5.

ABSTRACT: Hepatitis B virus (HBV) is a leading cause of liver‑related cancer. Progress has been made on the study of microRNA (miRNA or miR) function in HBV‑related liver cancer. Hence, the objective of the present study was to determine the role and functional mechanism of miR‑1271‑5p in HBV‑associated liver cancer. miR‑1271‑5p and aquaporin 5 (AQP5) expression at the mRNA level were measured by reverse transcription‑quantitative PCR (RT‑qPCR). The levels of hepatitis B e‑antigen (HBeAg), hepatitis B surface antigen (HBsAg) and HBV DNA were assessed by ELISA or qPCR. Cell viability, apoptosis, migration and invasion were detected by Cell Counting Kit‑8, flow cytometry or Transwell assay. The interaction of miR‑1271‑5p and AQP5 was predicted by TargetScan, and verified by dual‑luciferase reporter as

SUBMITTER: Li Z 

PROVIDER: S-EPMC7986005 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature