Comparison of ¹³ C MRI of hyperpolarized [1-¹³ C]pyruvate and lactate with the corresponding mass spectrometry images in a murine lymphoma model.

ABSTRACT:

Purpose

To compare carbon-13 (¹³ C) MRSI of hyperpolarized [1-¹³ C]pyruvate metabolism in a murine tumor model with mass spectrometric (MS) imaging of the corresponding tumor sections in order to cross validate these metabolic imaging techniques and to investigate the effects of pyruvate delivery and tumor lactate concentration on lactate labeling.

Methods

[1-¹³ C]lactate images were obtained from tumor-bearing mice, following injection of hyperpolarized [1-¹³ C]pyruvate, using a single-shot 3D ¹³ C spectroscopic imaging sequence in vivo and using desorption electrospray ionization MS imaging of the corresponding rapidly frozen tumor sections ex vivo. The images were coregistered, and levels of association were determine

SUBMITTER: Fala M 

PROVIDER: S-EPMC7986146 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature