Long-term stabilization of DNA at room temperature using a one-step microwave assisted process.

ABSTRACT: Long-term stabilization of DNA is needed for forensic, clinical, in-field operations and numerous other applications. Although freezing (<-20 °C) and dry storage are currently the preferential methods for long-term storage, a noticeable pre-analytical degradation of DNA over time, upfront capital investment and recurring costs have demonstrated a need for an alternative long-term room-temperature preservation method. Herein, we report a novel, fast (~5 min) silica sol-gel preparation method using a standard microwave-initiated polymerization reaction amenable to stabilization of DNA. The method involves use of one chemical, tetramethoxy silane (TMOS) and eliminates the use of alcohol as co-solvent and catalysts such as acids. In addition, the process involves minimal technical expertise, t

SUBMITTER: Narvaez Villarrubia CW 

PROVIDER: S-EPMC7986179 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature