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Production and monitoring of biomass and fucoxanthin with brown microalgae under outdoor conditions.

ABSTRACT: The effect of light on biomass and fucoxanthin (Fx) productivities was studied in two microalgae, Tisochrysis lutea and Phaeodactylum tricornutum. High and low biomass concentrations (1.1 and 0.4 g L^-1 ) were tested in outdoor pilot-scale flat-panel photobioreactors at semi-continuous cultivation mode. Fluorescence spectroscopy coupled with chemometric modeling was used to develop prediction models for Fx content and for biomass concentration to be applied for both microalgae species. Prediction models showed high R² for cell concentration (.93) and Fx content (.77). Biomass productivity was lower for high biomass concentration than low biomass concentration, for both microalgae (1.1 g L^-1 : 75.66 and 98.14 mg L^-1 d^-1 , for T. lutea and P. tricornutum, respectively; 0.4 g L^-1 : 129.9 and 158.47 mg L^-1 d^-1 , T. lutea and P. tricornutum). The same trend was observed in Fx productivity (1.1 g L^-1 : 1.14 and 1.41 mg L^-1 d^-1 , T. lutea and P. tricornutum; 0.4 g L^-1 : 2.09 and 1.73 mg L^-1 d^-1 , T. lutea and P. tricornutum). These results show that biomass and Fx productivities can be set by controlling biomass concentration under outdoor conditions and can be predicted using fluorescence spectroscopy. This monitoring tool opens new possibilities for online process control and optimization.

SUBMITTER: Gao F

PROVIDER: S-EPMC7986402 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature

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Production and monitoring of biomass and fucoxanthin with brown microalgae under outdoor conditions.

Gao Fengzheng F Sá Marta M Teles Cabanelas Itd Iago I Wijffels René H RH Barbosa Maria J MJ

Biotechnology and bioengineering 20201231 3

The effect of light on biomass and fucoxanthin (Fx) productivities was studied in two microalgae, Tisochrysis lutea and Phaeodactylum tricornutum. High and low biomass concentrations (1.1 and 0.4 g L<sup>-1</sup> ) were tested in outdoor pilot-scale flat-panel photobioreactors at semi-continuous cultivation mode. Fluorescence spectroscopy coupled with chemometric modeling was used to develop prediction models for Fx content and for biomass concentration to be applied for both microalgae species. ...[more]

PMID: 33325031

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Project description:Microalgae represent a sustainable source of biomass that can be exploited for pharmaceutical, nutraceutical, cosmetic applications, as well as for food, feed, chemicals, and energy. To make microalgae applications economically competitive and maximize their positive environmental impact, it is however necessary to optimize productivity when cultivated at a large scale. Independently from the final product, this objective requires the optimization of biomass productivity and thus of microalgae ability to exploit light for CO2 fixation. Light is a highly variable environmental parameter, continuously changing depending on seasons, time of the day, and weather conditions. In microalgae large scale cultures, cell self-shading causes inhomogeneity in light distribution and, because of mixing, cells move between different parts of the culture, experiencing abrupt changes in light exposure. Microalgae evolved multiple regulatory mechanisms to deal with dynamic light conditions that, however, are not adapted to respond to the complex mixture of natural and artificial fluctuations found in large-scale cultures, which can thus drive to oversaturation of the photosynthetic machinery, leading to consequent oxidative stress. In this work, the present knowledge on the regulation of photosynthesis and its implications for the maximization of microalgae biomass productivity are discussed. Fast mechanisms of regulations, such as Non-Photochemical-Quenching and cyclic electron flow, are seminal to respond to sudden fluctuations of light intensity. However, they are less effective especially in the 1-100 s time range, where light fluctuations were shown to have the strongest negative impact on biomass productivity. On the longer term, microalgae modulate the composition and activity of the photosynthetic apparatus to environmental conditions, an acclimation response activated also in cultures outdoors. While regulation of photosynthesis has been investigated mainly in controlled lab-scale conditions so far, these mechanisms are highly impactful also in cultures outdoors, suggesting that the integration of detailed knowledge from microalgae large-scale cultivation is essential to drive more effective efforts to optimize biomass productivity.

Dataset Information

Production and monitoring of biomass and fucoxanthin with brown microalgae under outdoor conditions.

Publications

Production and monitoring of biomass and fucoxanthin with brown microalgae under outdoor conditions.

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