Specific depletion of resident microglia in the early stage of stroke reduces cerebral ischemic damage.

ABSTRACT:

Background

Ischemia can induce rapid activation of microglia in the brain. As key immunocompetent cells, reactive microglia play an important role in pathological development of ischemic stroke. However, the role of activated microglia during the development of ischemia remains controversial. Thus, we aimed to investigate the function of reactive microglia in the early stage of ischemic stroke.

Methods

A Rose Bengal photothrombosis model was applied to induce targeted ischemic stroke in mice. CX3CR1^CreER:R26^iDTR mice were used to specifically deplete resident microglia through intragastric administration of tamoxifen (Ta) and intraperitoneal injection of diphtheria toxin (DT). At day 3 after ischemic stroke, behavioral tests were performed. After that,

SUBMITTER: Li T 

PROVIDER: S-EPMC7986495 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature