In vitro assessment of two novel Cellulases from Trabulsiella odontotermitis for agricultural waste utilization.

ABSTRACT:

Background

The production of agricultural wastes still growing as a consequence of the population growing. However, the majority of these residues are under-utilized due their chemical composition, which is mainly composed by cellulose. Actually, the search of cellulases with high efficiency to degrade this carbohydrate remains as the challenge. In the present experiment, two genes encoding an endoglucanase (EC 3.2.1.4) and β-glucosidase (EC 3.2.1.21) were overexpressed in Escherichia coli and their recombinant enzymes (egl-FZYE and cel-FZYE, respectively) characterized. Those genes were found in Trabulsiella odontermitis which was isolated from the gut of termite Heterotermes sp. Additionally, the capability to release sugars from agricultural wastes was evaluated in both enzymes,

SUBMITTER: Arevalos-Sanchez MM 

PROVIDER: S-EPMC7986525 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature