Phage susceptibility testing and infectious titer determination through wide-field lensless monitoring of phage plaque growth.

ABSTRACT: The growing number of drug-resistant bacterial infections worldwide is driving renewed interest in phage therapy. Based on the use of a personalized cocktail composed of highly specific bacterial viruses, this therapy relies on a range of tests on agar media to determine the most active phage on a given bacterial target (phage susceptibility testing), or to isolate new lytic phages from an environmental sample (enrichment of phage banks). However, these culture-based techniques are still solely interpreted through direct visual detection of plaques. The main objective of this work is to investigate computer-assisted methods in order to ease and accelerate diagnosis in phage therapy but also to study phage plaque growth kinetics. For this purpose, we designed a custom wide-field lensless im

SUBMITTER: Perlemoine P 

PROVIDER: S-EPMC7987195 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature