Identification of extracellular matrix proteins secreted by human dermal fibroblasts cultured in 3D electrospun scaffolds.

ABSTRACT: The appreciation that cell interactions in tissues is dependent on their three dimensional (3D) distribution has stimulated the development of 3D cell culture models. We constructed an artificial 3D tumour by culturing human breast cancer JIMT-1 cells and human dermal fibroblasts (HDFs) in a 3D network of electrospun polycaprolactone fibres. Here, we investigate ECM components produced by the cells in the artificial 3D tumour, which is an important step in validating the model. Immunostaining and confocal fluorescence microscopy show that the ECM proteins fibronectin, collagen I, and laminin are deposited throughout the entire 3D structure. Secreted soluble factors including matrix metalloproteinases (MMPs) and interleukine-6 (IL-6) were analysed in collected medium and were found to be ma

SUBMITTER: Malakpour-Permlid A 

PROVIDER: S-EPMC7988018 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature