The nickel-chelator dimethylglyoxime inhibits human amyloid beta peptide in vitro aggregation.

ABSTRACT: One of the hallmarks of the most common neurodegenerative disease, Alzheimer's disease (AD), is the extracellular deposition and aggregation of Amyloid Beta (Aβ)-peptides in the brain. Previous studies have shown that select metal ions, most specifically copper (Cu) and zinc (Zn) ions, have a synergistic effect on the aggregation of Aβ-peptides. In the present study, inductively coupled plasma mass spectrometry (ICP-MS) was used to determine the metal content of a commercial recombinant human Aβ₄₀ peptide. Cu and Zn were among the metals detected; unexpectedly, nickel (Ni) was one of the most abundant elements. Using a fluorescence-based assay, we found that Aβ₄₀ peptide in vitro aggregation was enhanced by addition of Zn²⁺ and Ni²⁺, and Ni²

SUBMITTER: Benoit SL 

PROVIDER: S-EPMC7988135 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature