Standardization of esophageal adenocarcinoma in vitro model and its applicability for model drug testing.

ABSTRACT: FLO-1 cell line represents an important tool in esophageal adenocarcinoma (EAC) research as a verified and authentic cell line to study the disease pathophysiology and antitumor drug screenings. Since in vitro characteristics of cells depend on the microenvironment and culturing conditions, we performed a thorough characterization of the FLO-1 cell line under different culturing conditions with the aim of (1) examining the effect of serum-free growth medium and air-liquid interface (A-L) culturing, which better reflect physiological conditions in vivo and (2) investigating the differentiation potential of FLO-1 cells to mimic the properties of the in vivo esophageal epithelium. Our study shows that the composition of the media influenced the morphological, ultrastructural and molecular cha

SUBMITTER: Tratnjek L 

PROVIDER: S-EPMC7988140 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature