Evaluation of the gut microbiome in association with biological signatures of inflammation in murine polytrauma and shock.

ABSTRACT: Severe injuries are frequently accompanied by hemorrhagic shock and harbor an increased risk for complications. Local or systemic inflammation after trauma/hemorrhage may lead to a leaky intestinal epithelial barrier and subsequent translocation of gut microbiota, potentially worsening outcomes. To evaluate the extent with which trauma affects the gut microbiota composition, we performed a post hoc analysis of a murine model of polytrauma and hemorrhage. Four hours after injury, organs and plasma samples were collected, and the diversity and composition of the cecal microbiome were evaluated using 16S rRNA gene sequencing. Although cecal microbial alpha diversity and microbial community composition were not found to be different between experimental groups, norepinephrine support in shock

SUBMITTER: Appiah SA 

PROVIDER: S-EPMC7988149 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature