Selective time-dependent changes in activity and cell-specific gene expression in human postmortem brain.

ABSTRACT: As a means to understand human neuropsychiatric disorders from human brain samples, we compared the transcription patterns and histological features of postmortem brain to fresh human neocortex isolated immediately following surgical removal. Compared to a number of neuropsychiatric disease-associated postmortem transcriptomes, the fresh human brain transcriptome had an entirely unique transcriptional pattern. To understand this difference, we measured genome-wide transcription as a function of time after fresh tissue removal to mimic the postmortem interval. Within a few hours, a selective reduction in the number of neuronal activity-dependent transcripts occurred with relative preservation of housekeeping genes commonly used as a reference for RNA normalization. Gene clustering indicated

SUBMITTER: Dachet F 

PROVIDER: S-EPMC7988150 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature