Nucleosome Clutches are Regulated by Chromatin Internal Parameters.

ABSTRACT: Nucleosomes cluster together when chromatin folds in the cell to form heterogeneous groups termed "clutches". These structural units add another level of chromatin regulation, for example during cell differentiation. Yet, the mechanisms that regulate their size and compaction remain obscure. Here, using our chromatin mesoscale model, we dissect clutch patterns in fibers with different combinations of nucleosome positions, linker histone density, and acetylation levels to investigate their role in clutch regulation. First, we isolate the effect of each chromatin parameter by studying systems with regular nucleosome spacing; second, we design systems with naturally-occurring linker lengths that fold onto specific clutch patterns; third, we model gene-encoding fibers to understand how these c

SUBMITTER: Portillo-Ledesma S 

PROVIDER: S-EPMC7988292 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature