Improving dairy performance through molecular characterization of SREBP-1 gene in Sarda sheep breed.

ABSTRACT: This research has two aims: (i) to characterize the coding sequence of the SREBP-1 gene in dairy sheep in order to investigate possible relationships between single nucleotide polymorphisms (SNPs) and milk traits; and (ii) to investigate possible relationship between SREBP-1 gene expression and nucleotide variation. Four hundred adult and multiparous lactating Sarda breed ewes were selected from two farms. Milk samples were collected from Day 30 to Day 150 of lactation to determine the mean yield, somatic cell count, lactose, fat, and protein content of the milk. RNA was extracted from the milk samples, after which the SREBP-1 gene coding regions were amplified and sequenced to scan mutations. Whilst eight SNPs were identified, none had statistically significant associ

SUBMITTER: Carcangiu V 

PROVIDER: S-EPMC7988322 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature