Solar photon-Fenton process eliminates free plasmid DNA harboring antimicrobial resistance genes from wastewater.

ABSTRACT: This work aimed to assess the elimination and inactivation of resistance-conferring plasmids (RCPs) present in suspension in secondary wastewater by solar photo-Fenton as these are important vectors for the dissemination of antimicrobial resistance. Experiments were performed in synthetic secondary wastewater (SWW) and municipal wastewater treatment plant effluent (MWWTPE). Solar photo-Fenton (50 mg L^-1 of H₂O₂ and 30 mg L^-1 of Fe²⁺) was carried out for 60 min at neutral pH by applying the intermittent iron addition strategy. The removal of RCPs was assessed by Real-Time Polymerase Chain Reaction (qPCR). The transformation of competent non-resistant E. coli was used to evaluate the inactivation of target RCPs harboring antibiotic resis

SUBMITTER: Vilela PB 

PROVIDER: S-EPMC7988504 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature