Glycosyltransferase Jhp0106 (PseE) contributes to flagellin maturation in Helicobacter pylori.

ABSTRACT:

Background

Flagella-mediated motility is both a crucial virulence determinant of Helicobacter pylori and a factor associated with gastrointestinal diseases. Flagellar formation requires flagellins to be glycosylated with pseudaminic acid (Pse), a process that has been extensively studied. However, the transfer of Pse to flagellins remains poorly understood. Therefore, the aim of this study is to characterize a putative glycosyltransferase jhp0106 in flagellar formation.

Materials and methods

Western blotting and chemical deglycosylation were performed to examine FlaA glycosylation. Protein structural analyses were executed to identify the active site residues of Jhp0106, while the Jhp0106-FlaA interaction was examined using a bacterial two-hybrid assay. Lastly, site-directed

SUBMITTER: Yang KY 

PROVIDER: S-EPMC7988653 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature