Hybrid genome de novo assembly with methylome analysis of the anaerobic thermophilic subsurface bacterium Thermanaerosceptrum fracticalcis strain DRI-13^T.

ABSTRACT:

Background

There is a dearth of sequenced and closed microbial genomes from environments that exceed > 500 m below level terrestrial surface. Coupled with even fewer cultured isolates, study and understanding of how life endures in the extreme oligotrophic subsurface environments is greatly hindered. Using a de novo hybrid assembly of Illumina and Oxford Nanopore sequences we produced a circular genome with corresponding methylome profile of the recently characterized thermophilic, anaerobic, and fumarate-respiring subsurface bacterium, Thermanaerosceptrum fracticalcis, strain DRI-13^T to understand how this microorganism survives the deep subsurface.

Results

The hybrid assembly produced a single circular genome of 3.8 Mb in length with an overall GC content of 45%

SUBMITTER: Murphy TR 

PROVIDER: S-EPMC7988955 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature