High throughput sequencing from Angolan citrus accessions discloses the presence of emerging CTV strains.

ABSTRACT:

Background

Citrus industry is worldwide dramatically affected by outbreaks of Citrus tristeza virus (CTV). Controls should be applied to nurseries, which could act as diversity hotspots for CTV. Early detection and characterization of dangerous or emerging strains of this virus greatly help to prevent outbreaks of disease. This is particularly relevant in those growing regions where no dedicated certification programs are currently in use.

Methods

Double-stranded RNA extracted from Citrus spp. samples, collected in two locations in Angola, were pooled and submitted to a random-primed RNA-seq. This technique was performed to acquire a higher amount of data in the survey, before the amplification and sequencing of genes from single plants. To confirm the CTV infection in indiv

SUBMITTER: Pais da Cunha AT 

PROVIDER: S-EPMC7988965 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature