AMD1 upregulates hepatocellular carcinoma cells stemness by FTO mediated mRNA demethylation.

ABSTRACT:

Background

S-adenosylmethionine decarboxylase proenzyme (AMD1) is a key enzyme involved in the synthesis of spermine (SPM) and spermidine (SPD), which are associated with multifarious cellular processes. It is also found to be an oncogene in multiple cancers and a potential target for tumor therapy. Nevertheless, the role AMD1 plays in hepatocellular carcinoma (HCC) is still unknown.

Methods

HCC samples were applied to detect AMD1 expression and evaluate its associations with clinicopathological features and prognosis. Subcutaneous and orthotopic tumor mouse models were constructed to analyze the proliferation and metastasis of HCC cells after AMD1 knockdown or overexpression. Drug sensitive and tumor sphere assay were performed to investigate the effect of AMD1 on HCC cells

SUBMITTER: Bian X 

PROVIDER: S-EPMC7989706 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature