Gene mutations of esophageal squamous cell carcinoma based on next-generation sequencing.

ABSTRACT:

Background

Esophageal squamous cell carcinoma (ESCC) is one of the most aggressive cancers without effective therapy. To explore potential molecular targets in ESCC, we quantified the mutation spectrum and explored the relationship between gene mutation and clinicopathological characteristics and programmed death-ligand 1 (PD-L1) expression.

Methods

Between 2015 and 2019, 29 surgically resected ESCC tissues and adjacent normal tissues from the Fourth Hospital of Hebei Medical University were subjected to targeted next-generation sequencing. The expression levels of PD-L1 were detected by immunohistochemistry. Mutational signatures were extracted from the mutation count matrix by using non-negative matrix factorization. The relationship between detected genomic alterations an

SUBMITTER: Wang L 

PROVIDER: S-EPMC7990006 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature