Mechanistic differences underlying HIV latency in the gut and blood contribute to differential responses to latency-reversing agents.

ABSTRACT:

Objective

While latently HIV-infected cells have been described in the blood, it is unclear whether a similar inducible reservoir exists in the gut, where most HIV-infected cells reside. Tissue-specific environments may contribute to differences in the mechanisms that govern latent HIV infection and amenability to reactivation. We sought to determine whether HIV-infected cells from the blood and gut differ in their responses to T-cell activation and mechanistically distinct latency reversing agents (LRAs).

Design

Cross sectional study using samples from HIV-infected individuals (n = 11).

Methods

Matched peripheral blood mononuclear cells (PBMC) and dissociated total cells from rectum ± ileum were treated ex vivo for 24 h with anti-CD3/CD28 or LRAs in the presence of a

SUBMITTER: Telwatte S 

PROVIDER: S-EPMC7990078 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature