Quantification by Luminescence Tracking of Red Emissive Gold Nanoparticles in Cells.

ABSTRACT: Optical microscopy techniques are ideal for live cell imaging for real-time nanoparticle tracking of nanoparticle localization. However, the quantification of nanoparticle uptake is usually evaluated by analytical methods that require cell isolation. Luminescent labeling of gold nanoparticles with transition metal probes yields particles with attractive photophysical properties, enabling cellular tracking using confocal and time-resolved microscopies. In the current study, gold nanoparticles coated with a red-luminescent ruthenium transition metal complex are used to quantify and track particle uptake and localization. Analysis of the red-luminescence signal from particles is used as a metric of cellular uptake, which correlates to total cellular gold and ruthenium content, independently m

SUBMITTER: Dosumu AN 

PROVIDER: S-EPMC7990080 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature