Wnt16 Elicits a Protective Effect Against Fractures and Supports Bone Repair in Zebrafish.

ABSTRACT: Bone homeostasis is a dynamic, multicellular process that is required throughout life to maintain bone integrity, prevent fracture, and respond to skeletal damage. WNT16 has been linked to bone fragility and osteoporosis in human genome wide-association studies, as well as the functional hematopoiesis of leukocytes in vivo. However, the mechanisms by which WNT16 promotes bone health and repair are not fully understood. In this study, CRISPR-Cas9 was used to generate mutant zebrafish lacking Wnt16 (wnt16 ^-/- ) to study its effect on bone dynamically. The wnt16 mutants displayed variable tissue mineral density (TMD) and were susceptible to spontaneous fractures and the accumulation of bone calluses at an early age. Fractures were induced i

SUBMITTER: McGowan LM 

PROVIDER: S-EPMC7990157 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature