Ontology highlight
ABSTRACT:
SUBMITTER: Knox HL
PROVIDER: S-EPMC7990684 | biostudies-literature | 2021 Apr
REPOSITORIES: biostudies-literature
Knox Hayley L HL Chen Percival Yang-Ting PY Blaszczyk Anthony J AJ Mukherjee Arnab A Grove Tyler L TL Schwalm Erica L EL Wang Bo B Drennan Catherine L CL Booker Squire J SJ
Nature chemical biology 20210118 4
Tryptophan 2C methyltransferase (TsrM) methylates C2 of the indole ring of L-tryptophan during biosynthesis of the quinaldic acid moiety of thiostrepton. TsrM is annotated as a cobalamin-dependent radical S-adenosylmethionine (SAM) methylase; however, TsrM does not reductively cleave SAM to the universal 5'-deoxyadenosyl 5'-radical intermediate, a hallmark of radical SAM (RS) enzymes. Herein, we report structures of TsrM from Kitasatospora setae, which are the first structures of a cobalamin-dep ...[more]