Changes in DNA methylation accompany changes in gene expression during chondrocyte hypertrophic differentiation in vitro.

ABSTRACT: During osteoarthritis (OA), articular chondrocytes undergo phenotypic changes that resemble developmental patterns characteristic of growth plate chondrocytes. These phenotypic alterations lead to a hypertrophy-like phenotype characterized by altered production of extracellular matrix constituents and increased collagenase activity, which, in turn, results in cartilage destruction in OA disease. Recent studies have shown that the phenotypic instability and dysregulated gene expression in OA are associated with changes in DNA methylation patterns. Subsequent efforts have aimed to identify changes in DNA methylation with functional impact in OA disease, to potentially uncover therapeutic targets. Here, we paired an in vitro 3D/pellet culture system that mimics chondrocyte hypertrophy with RN

SUBMITTER: Singh P 

PROVIDER: S-EPMC7990741 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature