Protein manipulation using single copies of short peptide tags in cultured cells and in <i>Drosophila melanogaster</i>.

ABSTRACT: Cellular development and function rely on highly dynamic molecular interactions among proteins distributed in all cell compartments. Analysis of these interactions has been one of the main topics in cellular and developmental research, and has been mostly achieved by the manipulation of proteins of interest (POIs) at the genetic level. Although genetic strategies have significantly contributed to our current understanding, targeting specific interactions of POIs in a time- and space-controlled manner or analysing the role of POIs in dynamic cellular processes, such as cell migration or cell division, would benefit from more-direct approaches. The recent development of specific protein binders, which can be expressed and function intracellularly, along with advancement in synthetic biology,

SUBMITTER: Vigano MA 

PROVIDER: S-EPMC7990863 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature