Prediction of Function Determining and Buried Residues Through Analysis of Saturation Mutagenesis Datasets.

ABSTRACT: Mutational scanning can be used to probe effects of large numbers of point mutations on protein function. Positions affected by mutation are primarily at either buried or at exposed residues directly involved in function, hereafter designated as active-site residues. In the absence of prior structural information, it has not been easy to distinguish between these two categories of residues. We curated and analyzed a set of twelve published deep mutational scanning datasets. The analysis revealed differential patterns of mutational sensitivity and substitution preferences at buried and exposed positions. Prediction of buried-sites solely from the mutational sensitivity data was facilitated by incorporating predicted sequence-based accessibility values. For active-site residues we observed m

SUBMITTER: Bhasin M 

PROVIDER: S-EPMC7991590 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature