Three-Dimensional Architecture of Glomerular Endothelial Cells Revealed by FIB-SEM Tomography.

ABSTRACT: Focused-ion beam-scanning electron microscopic (FIB-SEM) tomography enables easier acquisition of a series of ultrastructural, sectional images directly from resin-embedded biological samples. In this study, to clarify the three-dimensional (3D) architecture of glomerular endothelial cells (GEnCs) in adult rats, we manually extracted GEnCs from serial FIB-SEM images and reconstructed them on an Amira reconstruction software. The luminal and basal surface structures were clearly visualized in the reconstructed GEnCs, although only the luminal surface structures could be observed by conventional SEM. The luminal surface visualized via the reconstructed GEnCs was quite similar to that observed through conventional SEM, indicating that 3D reconstruction could be performed with high accuracy. T

SUBMITTER: Kawasaki Y 

PROVIDER: S-EPMC7991748 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature