Extensive Sub-RPE Complement Deposition in a Nonhuman Primate Model of Early-Stage Diabetic Retinopathy.

ABSTRACT:

Purpose

This study aims to reveal retinal abnormities in a spontaneous diabetic nonhuman primate model and explore the mechanism of featured injuries.

Methods

Twenty-eight cynomolgus monkeys were identified to suffer from spontaneous type 2 diabetes from a colony of more than eight-hundred aged monkeys, and twenty-six age-matched ones were chosen as controls. Their blood biochemistry profiles were determined and retinal changes were examined by multimodal imaging, hematoxylin and eosin staining, and immunofluorescence. Retinal pigment epithelium (RPE) cells were further investigated by RNA sequencing and computational analyses.

Results

These diabetic monkeys were characterized by early retinal vascular and neural damage and dyslipidemia. The typical acellular capillar

SUBMITTER: Fan S 

PROVIDER: S-EPMC7991921 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature