Epigenetic Regulation of Filaggrin Gene Expression in Human Epidermal Keratinocytes.

ABSTRACT:

Background

Loss-of-function mutations in the filaggrin gene (FLG), which encodes an epidermal protein crucial for the formation of a functional skin barrier, have been identified as a major predisposing factor in the etiopathogenesis of atopic dermatitis (AD). Recent reports of relatively low frequencies of FLG-null mutations among specific ethnic groups with AD necessitated analysis of the epigenetic regulation which may control FLG expression without altering its DNA sequence.

Objective

The study aimed to identify DNA methylation-dependent regulation of FLG expression.

Methods

Quantitative polymerase chain reaction was performed to determine the restoration of FLG mRNA expression in normal human epidermal keratinocyte (NHEK) cells after treatment with epigenetic mod

SUBMITTER: Lee J 

PROVIDER: S-EPMC7992546 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature