TGF-β1 modulates temozolomide resistance in glioblastoma via altered microRNA processing and elevated MGMT.

ABSTRACT:

Background

Our previous studies have indicated that miR-198 reduces cellular methylguanine DNA methyltransferase (MGMT) levels to enhance temozolomide sensitivity. Transforming growth factor beta 1 (TGF-β1) switches off miR-198 expression by repressing K-homology splicing regulatory protein (KSRP) expression in epidermal keratinocytes. However, the underlying role of TGF-β1 in temozolomide resistance has remained unknown.

Methods

The distribution of KSRP was detected by western blotting and immunofluorescence. Microarray analysis was used to compare the levels of long noncoding RNAs (lncRNAs) between TGF-β1-treated and untreated cells. RNA immunoprecipitation was performed to verify the relationship between RNAs and KSRP. Flow cytometry and orthotopic and subcutaneous xenogr

SUBMITTER: Nie E 

PROVIDER: S-EPMC7992894 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature