Critical role of mass spectrometry proteomics in tear biomarker discovery for multifactorial ocular diseases (Review).

ABSTRACT: The tear film is a layer of body fluid that maintains the homeostasis of the ocular surface. The superior accessibility of tears and the presence of a high concentration of functional proteins make tears a potential medium for the discovery of non‑invasive biomarkers in ocular diseases. Recent advances in mass spectrometry (MS) have enabled determination of an in‑depth proteome profile, improved sensitivity, faster acquisition speed, proven variety of acquisition methods, and identification of disease biomarkers previously lacking in the field of ophthalmology. The use of MS allows efficient discovery of tear proteins, generation of reproducible results, and, more importantly, determines changes of protein quantity and post‑translation modifications in microliter samples. The present revie

SUBMITTER: Ma JYW 

PROVIDER: S-EPMC7992922 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature