The influence of obesity on folate status, DNA methylation and cancer-related gene expression in normal breast tissues from premenopausal women.

ABSTRACT: Epidemiological studies have established obesity as a critical risk factor for postmenopausal breast cancer (post-BC), whereas a reverse association holds prior to menopause. A significant scientific gap exists in understanding the mechanism(s) underpinning this epidemiological phenomenon, particularly the reverse association between obesity and premenopausal breast cancer (pre-BC). This study aimed to understand how folate metabolism and DNA methylation inform the association between obesity and pre-BC. Fifty normal breast tissue samples were collected from premenopausal women who underwent reduction mammoplasty. We modified the Lactobacillus Casei microbiological folate assay and measured folate levels in our breast tissue samples. The DNA methylation of LINE-1, a biomarker

SUBMITTER: Frederick AM 

PROVIDER: S-EPMC7993144 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature